Equipment

The College of Science and Mathematics believes in providing students with research-grade, state-of-the-art equipment, both for learning and for research. We are always expanding and upgrading our suite of equipment, so our equipment list is not exhaustive.

  • The BiologGEN III MicroStation System rapidly and accurately identifies about 3,000 species of aerobic and anaerobic Bacteria, Yeasts, and Fungi. The systems phenotypic technology provides valuable information on the properties of strains, in addition to a species-level identification. The systems technology identifies environmental and pathogenic microorganisms by producing a characteristic pattern, or “metabolic fingerprint”, from individual test reactions performed within a 96 well microplate. Culture suspensions are tested with a panel of pre-selected assays, then incubated, read and compared to available databases.
  • Photo of the Biacore X100 SPR BiosensorOur workhorse Bioacore biosensor facilitates kinetic and affinity analysis for intermolecular interactions using the established surface plasmon resonance (SPR) technology. National Science Foundation )NSF) logoThe instrument is useful for a wide range of biological molecules and conditions, e.g. temperature control down to 4° C. Its sensitivity allows for analysis of small molecules down to 100 Da. Partially funded by NSF.
  • Using biolayer interferometry (BLI), the FortéBio Octet QK Biosensor allows for rapid, real-time, label-free kinetic analysis of binding between biomacromolecules. By analyzing the shift in the interference pattern of white light that results from one molecule binding another on disposable sensors, we can easily assign rate and affinity constants. National Institutes of Health (NIH)Kennesaw State is a leading publisher of BLI data Funded jointly by the National Institutes of Health (NIH) and the College of Science and Mathematics, our instrument and expertise are available to both internal and external scientists. Partially funded by NIH.
  • Photo of the Jasco 1500 Spectropolarimeter equipmentAll Jasco 1500 Spectropolarimeter (circular dichroism instrument) questions, training & scheduling inquiries, should be coordinated with the equipment coordinator, Thomas Leeper.

    Circular dichroism (CD) is well known as a powerful probe of molecular conformations. It is a manifestation of the optical activity of chiral chromophores. CD can be used to measure the optical properties of small molecules. Another good use of CD in biochemistry is the estimation of secondary structure in proteins. This is possible because the CD of the peptide bond region absorbance is highly sensitive to the Ramachandran angles of amino acid residues, allowing clean discrimination between well-defined structural features such as alpha helices and beta strands, which have different and well defined Ramachandran stability valleys. This is not the case for conformations lumped together as ‘random coil.’ Hence CD as a measure of secondary structure is typically descriptive rather than quantitatively accurate, although reasonable estimates of alpha helical and beta sheet content can be made.

    Circular dichroism is a very powerful probe of changes in protein conformation. For example, it is possible to observe small changes in helix content using difference methods, and effects of mutation and protein-protein interactions can be studied. These measurements are relatively easy to carry out and produce reliable, easily interpreted results. Studies of protein conformation rely on the absorbance of the chiral peptide bond region and are the result of electric and magnetic coupling of light to transitions.

  • Listed below are the various Spectroscopy Equipment housed within the College of Science and Mathematics. Click on the equipment names to view more information about that specific equipment.

    • Picomaster TCSPC Spectrometer
      Made by PTI, the instrument is currently equipped with excitation sources at 473, 375, 310 and 280 nm. The instrument can measure fluorescence decays down to 10 picoseconds, and can resolve multiple components by iterative fitting, including convolution with the exciting pulse shape. The T-2B Steady state enhancement module includes a high intensity continuous xenon source, an excitation monochromator, and a multi-mode PMT detector which can selected by turning a mirror. This allows high quality excitation and emission spectra to be obtained. The instrument is useful for FRET experiments, and can perform high quality fluorescence polarization experiments. Funded by NSF MRI.
    • Electron Paramagnetic Resonance
      The Electron paramagnetic resonance (EPR) spectrometer is a Bruker EMX running at X band (9.2GHz), equipped with an ER 083 electromagnet capable of reaching fields of 13 kG. Available cryogenics allow studies down to a few degrees above the boiling point of liquid helium, 4.2K. This allows studies of rapidly relaxing metal centers. EPR is a spectroscopic technique that provides information by detecting transitions between electron spin states. It is useful for studying molecules with unpaired electrons, including free radicals and metal centers. Paramagnetic probes (spin labels) and spin trapping reagents have extended its usefulness greatly.
  • The TA Instruments Nano ITC isothermal titration calorimetry is designed to provide maximum sensitivity and flexibility for the study of biomolecular binding. It allows determination of affinity and thermodynamic constants in solution. Our TA Instruments Nano ITC works in less than 200 μl of solution and can measure heat changes of less than 0.1 μJ. The instrument is also useful for studying enzyme kinetics and stoichiometry. This equipment is funded by NIH.

    For all inquiries, contact: Jonathan McMurry

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