The College of Science and Mathematics (CSM) believes in providing students, faculty and external users with research-grade, state-of-the-art equipment, both for learning and for research.
Biomolecular Interaction Analysis Facility
This facility is comprised of the following equipment:
- Biacore X100 SPR Biosensor
- Our workhorse Biacore X100 SPR Biosensor facilitates kinetic and affinity analysis for intermolecular interactions using the established surface plasmon resonance (SPR) technology. National Science Foundation )NSF) logoThe instrument is useful for a wide range of biological molecules and conditions, e.g. temperature control down to 4° C. Its sensitivity allows for analysis of small molecules down to 100 Da. Partially funded by NSF. For all inquiries, contact: Jonathan McMurry.
- ForteBio Octet Red96e Biosensor
- Using biolayer interferometry (BLI), the ForteBio Octet Red96e Biosensor allows for rapid, real-time, label-free kinetic analysis of binding between biomacromolecules. By analyzing the shift in the interference pattern of white light that results from one molecule binding another on disposable sensors, we can easily assign rate and affinity constants. Kennesaw State is a leading publisher of BLI data. Funded jointly by the National Institutes of Health (NIH) and the College of Science and Mathematics at Kennesaw State University. For all inquiries, contact: Jonathan McMurry.
- TA Instruments Nano ITC
- The TA Instruments Nano ITC isothermal titration calorimetry is designed to provide maximum sensitivity and flexibility for the study of biomolecular binding. It allows determination of affinity and thermodynamic constants in solution. Our TA Instruments Nano ITC works in less than 200 μl of solution and can measure heat changes of less than 0.1 μJ. The instrument is also useful for studying enzyme kinetics and stoichiometry. This equipment is funded by NIH. For all inquiries, contact: Thomas Leeper.
- Biacore X100 SPR Biosensor
Flow Cytometry and FACS Facility
This facility is comprised of the following equipment:
- Sony Biosciences SH800 Cell Sorter: The SH800 cell sorter is equipped with four lasers (405nm, 488nm, 561nm and 638nm) and six fluorescence detectors in addition to forward and backscatter detectors. This laser setup permits maximum flexibility in experimental design and allows easy discrimination between live cell labels such as GFP and RFP or Cherry. Disposable sorting chips featuring microfluidic technology are used to combine the sheath and sample lines. Fluidics, laser alignment, and sort stream calibration are all fully automated. The system can be run in analytical and preparative sort modes. Multiple sample tubes can be accommodated (0.5ml microfuge tube up to a 35ml centrifuge tube) and a wide variety of collection systems are available for cell sorting runs, including a 96 well-format collector that can be used in single cell sort mode. The entire instrument is housed in a custom-built Baker BSL class IIA biosafety cabinet, allowing the safe sorting of potentially hazardous samples. The system is controlled by proprietary Sony software and is very easy to set up and use. Please contact Martin Hudson for training and scheduling. This instrument was purchased via a NSF MRI grant.
- BD Acurri C6 Flow Cytometer: The Accuri C6 flow cytometer is equipped with 488nm and 647nm lasers, and four fluorescence detectors that can arranged in different modes via the use of interchangeable filters. Particle size and granularity are measured using forward and side-scatter detectors. The system is powered by BD software, and data can be exported in multiple file formats, including FCS-3 standard. The instrument is very easy to set up and run, and utilizes distilled water as the sheath fluid. Please contact Martin Hudson for training and scheduling. This instrument was purchased via a donation from the Georgia Research Alliance.
- FlowJo Flow Cytometry Analysis Software: An iMac equipped with FlowJo is available for off-line FACS and flow cytometry data analysis. This can be used for post-acquisition signal compensation, data analysis, and figure preparation for publication.
- Data Image Legend: Flow cytometry analysis of eGFP and mCherry transfected 293T cells. Data captured on the Sony SH800 cell sorter.
For all inquiries, contact: Martin Hudson (PI).
Glass Research Facility
The Glass Research Facility consists of two laboratories housed under the physics department at the Marietta campus: a High-Temperature Furnace Laboratory and a Thermal Laboratory.
- High-Temperature Furnace Laboratory: The furnace lab is in building G. The laboratory is equipped with Sentro-Tech ST-1600, and Thermo-Scientific Muffle-furnace, Mixer Mill MM 500, and an advanced digital glass cutter with diamond blades. The lab is equipped with a fume hood, storage cabinets, chemical storage, sink, eyewash, and shower. The sink, eyewash, and the shower are built with handicap access.
- Thermal Laboratory: This research laboratory is used for thermal analysis and X-Ray Diffraction studies. This lab is equipped with wall cabinets as well as countertop space with cabinets. The SDT Q600 Thermal Analyzer is housed in this lab and is on a special vibration-free table. The 554 800 RONTGENGERAT X-Ray apparatus is housed in this laboratory along with an optical microscope and a high precision scale. This laboratory is equipped with large N2, Ar, and dry air gas cylinders.
For all inquiries, contact: Dr. Kisa Ranasinghe.
KSU’s Histology Core Facility houses the latest specialized equipment needed to prepare high-quality histological sections for molecular, cellular, and anatomical based research projects. The facility is housed in the 300-square foot research Science laboratory and includes aLeica CM180 Cryostat, a Microm 650V Vibratome, and a Microm 360 Rotary microtome. This suite of equipment allows for the preparation of sections from frozen, paraffin, and agarose-embedded specimens, as well as both fixed and unfixed specimens.
In addition, the facility houses paraffin incubators, slide warmers, wet bench workspace, ample storage space, a fume hood, and a dedicated workstation. Please contact the Facility Coordinator for more information.
- Leica CM 1850 Cryostat: Minimum –35 °C chamber temperature, actively cooled quick freeze shelf with Peltier unit for freezing down to –60 °C. Splash-protected microtome, with two-speed motorized coarse feed. Smooth specimen orientation with automatic centering, and the lateral displacement feature of knife holder for disposable blades allows the use of the entire blade length without need to reposition the blade. A glass anti-roll guide integrated into the holder is automatically positioned with blade movement. Serial sections down to 3 μm are easily obtained. Advanced scheduling for use is required. Proper training is required for all researchers prior to use of this equipment. Funded by Georgia Research Alliance.
- Microm 650V Vibratome: The vibrating blade has been designed for sectioning fresh tissue, such as spinal cord, brain, and botanical specimens. This gentle sectioning method is also useful for sectioning many types of fixed tissues. Amplitude and frequency are set independently and optimized for each specimen. Two section thicknesses can be programmed between 1 and 1500 μm. Rapid return travel of the blade carriage (5 mm/sec) allows for efficient sectioning. Larger specimens can be accommodated by the 50mm horizontal programmable cutting window. Cutting speed is adjustable continuously between 0 – 50 mm/sec in 0.1 mm/sec-increments. Advanced scheduling for use is required. Proper training is required for all researchers prior to use of this equipment.
- Microm 360 Rotary microtome: Rotary microtomes are designed for sectioning of paraffin, semi-thin, and hard fixed specimens. The Microm 360 is touch pad keyboard controlled for thickness (trim: 5 to 500µms; section: 0.25 to 60µm), mode (single, interval, and continuous stroke), and speed with additional manual and/or foot pedal control options. Vertical specimen stroke is 64mm. Advanced scheduling for use is required. Proper training is required for all researchers prior to use of this equipment.
For all inquiries, contact: Anton Bryantsev (PI).
Joyce and Ira Pegues Memorial Greenhouse
The greenhouse is 600 square feet and has 8 wire-top benches that are 8 ft x 4 ft (256 square feet of bench space total). The greenhouse can be environmentally control; it has a full wall of negative pressure evaporative cooling and external shade cloth. This allows temperature in the greenhouse to stay below 30.5 degrees C maximum on the hottest summer days. There are two gas heaters in the greenhouse to keep it at desired temperature during the winter. The greenhouse has concrete floor. The greenhouse is located on the Kennesaw campus adjacent to the Science Laboratory building.
For all inquiries, please contact: Joel McNeal (PI).More greenhouse photos
Material Synthesis and Characterization LaboratoryThis research laboratory is housed under the physics department at the Marietta campus. The laboratory is equipped with synthesis and processing equipment and safety essentials required for the proposed work. Carbolite Gero box furnace, Mellen Microtherm 1250°C Split Hinged Tube Furnace, Arc melting furnace, Lab Hydraulic Manual Press w. ProtectionVacuum chamber with custom 0-70° temperature stage, SRS Model 860 1 mHz to 500 kHz digital lockin amplifier, Two Keithley 2182A nanovoltmeters Forward.
The Oasis - Outdoor classroom
The Oasis, a hands-on outdoor classroom, is one of most unique and innovative laboratory spaces on campus, that will enable biology students to study native Georgia plant life.
The Oasis was built in the unused courtyard space between the Science building, the Science Laboratory building and the Clendenin building. This initiative, which includes a water basin containing and surrounded by a diverse array of plant species, was funded by a generous donor, Dr. Joseph Cook, and named "The Oasis" by the College of Science and Mathematics students in the CSM Courtyard naming contest. Construction for Phase I was completed in the Summer of 2014.
BiologGEN III MicroStation SystemThe BiologGEN III MicroStation System rapidly and accurately identifies about 3,000 species of aerobic and anaerobic Bacteria, Yeasts, and Fungi. The systems phenotypic technology provides valuable information on the properties of strains, in addition to a species-level identification. The systems technology identifies environmental and pathogenic microorganisms by producing a characteristic pattern, or “metabolic fingerprint”, from individual test reactions performed within a 96 well microplate. Culture suspensions are tested with a panel of pre-selected assays, then incubated, read and compared to available databases.
Listed below are the various Spectroscopy Equipment housed within CSM:
- Electron Paramagnetic Resonance
- The Electron paramagnetic resonance (EPR) spectrometer is a Bruker EMX running at X band (9.2GHz), equipped with an ER 083 electromagnet capable of reaching fields of 13 kG. Available cryogenics allow studies down to a few degrees above the boiling point of liquid helium, 4.2K. This allows studies of rapidly relaxing metal centers. EPR is a spectroscopic technique that provides information by detecting transitions between electron spin states. It is useful for studying molecules with unpaired electrons, including free radicals and metal centers. Paramagnetic probes (spin labels) and spin trapping reagents have extended its usefulness greatly.
- Jasco 1500 Spectropolarimeter
- All Jasco 1500 Spectropolarimeter (circular dichroism instrument) questions, training
& scheduling inquiries, should be coordinated with the equipment coordinator, Thomas Leeper.
Circular dichroism (CD) is well known as a powerful probe of molecular conformations. It is a manifestation of the optical activity of chiral chromophores. CD can be used to measure the optical properties of small molecules. Another good use of CD in biochemistry is the estimation of secondary structure in proteins. This is possible because the CD of the peptide bond region absorbance is highly sensitive to the Ramachandran angles of amino acid residues, allowing clean discrimination between well-defined structural features such as alpha helices and beta strands, which have different and well defined Ramachandran stability valleys. This is not the case for conformations lumped together as ‘random coil.’ Hence CD as a measure of secondary structure is typically descriptive rather than quantitatively accurate, although reasonable estimates of alpha helical and beta sheet content can be made.
Circular dichroism is a very powerful probe of changes in protein conformation. For example, it is possible to observe small changes in helix content using difference methods, and effects of mutation and protein-protein interactions can be studied. These measurements are relatively easy to carry out and produce reliable, easily interpreted results. Studies of protein conformation rely on the absorbance of the chiral peptide bond region and are the result of electric and magnetic coupling of light to transitions.
- All Jasco 1500 Spectropolarimeter (circular dichroism instrument) questions, training & scheduling inquiries, should be coordinated with the equipment coordinator, Thomas Leeper.
- Picomaster TCSPC Spectrometer
- Made by PTI, the instrument is currently equipped with excitation sources at 473, 375, 310 and 280 nm. The instrument can measure fluorescence decays down to 10 picoseconds, and can resolve multiple components by iterative fitting, including convolution with the exciting pulse shape. The T-2B Steady state enhancement module includes a high intensity continuous xenon source, an excitation monochromator, and a multi-mode PMT detector which can selected by turning a mirror. This allows high quality excitation and emission spectra to be obtained. The instrument is useful for FRET experiments, and can perform high quality fluorescence polarization experiments. Funded by NSF MRI.
- Electron Paramagnetic Resonance