Microscopy Core Facility

  • Managing PI: Marcus Davis

    LSM 700 is in the inverted configuration to facilitate live cell imaging. Four solid-state lasers (405, 488, 555, and 639nm) are available. A key innovation of the LSM 700 system is the use of a variable secondary dichroic (VSD) beamsplitter which enables efficient yet rapid detection of up to four different color signals. National Science Foundation (NSF) MRIRather than requiring separate filters for each desired fluorophore, this system uses a sliding dichroic mirror to rapidly direct specific wavelengths to selected photodetectors. This technology allows use of the entire emission spectrum for imaging, while simultaneously preventing crosstalk between closely positioned fluorophore emission spectra. Microscope objectives are available to cover a broad range of sample sizes, thicknesses, densities, and opacities. Advanced scheduling for use is required.  Proper training in microscope, camera and software usage is required for all researchers on this equipment. Funded by NSF MRI.

  • Managing PI: Eric Albrecht

    Coupled to a DP71 12 million pixel CCD camera which is connected to a Dell Optiplex workstation. The microscope contains FITC (HQ480 (EX)/Q505LP(BS)/ HQ535(EM)) and TRITC (HQ535 (EX)/Q565LP(BS),HQ610(EM)) filter sets. Additional capabilities include phase and DIC imaging (40 and 100X). Advanced scheduling for use is required. Proper training in microscope, camera and software usage is required for all researchers on this equipment.

  • Managing PI: Martin Hudson

    Liquid light epifluorescence illumination, DIC optics, a high resolution/ high sensitivity camera (Qimaging Retiga EXi system), motorized Z-axis and state-of-the-art control software, featuring acquisition automation for multichannel imaging, a deconvolution module and measurement tools. The BX61 features 10x, 40x, 63x and 100x objectives. This microscope is often used for overnight imaging of development – advanced scheduling for use is required. Proper training in microscope, camera and software usage is required for all researchers on this equipment.

  • Managing PI: Martin Hudson

    Shares the Qimaging Retiga EXi system with the BX61. Advanced scheduling for use is required. Proper training in microscope, camera and software usage is required for all researchers on this equipment.

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