Histology Core Facility
Kennesaw State’s Histology Core Facility houses the latest specialized equipment needed to prepare high-quality histological sections for molecular, cellular, and anatomical based research projects. The facility is housed in the 300-square foot research Science laboratory and includes aLeica CM180 Cryostat, a Microm 650V Vibratome, and a Microm 360 Rotary microtome. This suite of equipment allows for the preparation of sections from frozen, paraffin, and agarose-embedded specimens, as well as both fixed and unfixed specimens.
In addition, the facility houses paraffin incubators, slide warmers, wet bench workspace, ample storage space, a fume hood, and a dedicated workstation. Please contact the relevant equipment PI, or the Facility PI for more information.
Leica CM1850 Cryostat
Managing PI: Lisa Ganser
Minimum –35 °C chamber temperature, actively cooled quick freeze shelf with Peltier unit for freezing down to –60 °C. Splash-protected microtome, with two-speed motorized coarse feed. Smooth specimen orientation with automatic centering, and the lateral displacement feature of knife holder for disposable blades allows the use of the entire blade length without need to reposition the blade. A glass anti-roll guide integrated into the holder is automatically positioned with blade movement. Serial sections down to 3 μm are easily obtained. Advanced scheduling for use is required. Proper training is required for all researchers prior to use of this equipment. Funded by Georgia Research Alliance.
Microm 650V Vibratome
Managing PI: Marcus Davis
The vibrating blade has been designed for sectioning fresh tissue, such as spinal cord, brain, and botanical specimens. This gentle sectioning method is also useful for sectioning many types of fixed tissues. Amplitude and frequency are set independently and optimized for each specimen. Two section thicknesses can be programmed between 1 and 1500 μm. Rapid return travel of the blade carriage (5 mm/sec) allows for efficient sectioning. Larger specimens can be accommodated by the 50mm horizontal programmable cutting window. Cutting speed is adjustable continuously between 0 – 50 mm/sec in 0.1 mm/sec-increments. Advanced scheduling for use is required. Proper training is required for all researchers prior to use of this equipment.
Microm 360 Rotary microtome
Managing PI: Marcus Davis
Rotary microtomes are designed for sectioning of paraffin, semi-thin, and hard fixed specimens. The Microm 360 is touch pad keyboard controlled for thickness (trim: 5 to 500µms; section: 0.25 to 60µm), mode (single, interval, and continuous stroke), and speed with additional manual and/or foot pedal control options. Vertical specimen stroke is 64mm. Advanced scheduling for use is required. Proper training is required for all researchers prior to use of this equipment.